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pet30b expression vectors  (Millipore)


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    Structured Review

    Millipore pet30b expression vectors
    Pet30b Expression Vectors, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30b+expression+vector/pet28a/pmc11166852-160-4-7
    Average 90 stars, based on 1 article reviews
    pet30b expression vectors - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Structural and Functional Investigation of the Ag(+)/Cu(+) Binding Domains of the Periplasmic Adaptor Protein SilB from Cupriavidus metallidurans CH34.
    Article Snippet: Silver ion resistance in bacteria mainly relies on efflux systems, and notably on tripartite efflux complexes involving a transporter from the resistance−nodulation−cell division (RND) superfamily, such as the SilCBA system from Cupriavidus metallidurans CH34.. The periplasmic adaptor protein SilB hosts two specific metal coordination sites, located in the N-terminal and C-terminal domains, respectively, that are believed to play a different role in the efflux mechanism and the trafficking of metal ions from the periplasm to the RND transporter.. On the basis of the known domain structure of periplasmic adaptor proteins, we designed different protein constructs derived from SilB domains with either one or two metal binding sites per protein chain.

    Article Title: Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB
    Article Snippet: The amplified PCR product was cloned in the pJET1.2/blunt vector (ThermoFisher Scientific) prior to sequencing. .. The lasB gene was then excised by digesting with Nde I and Xho I and cloned into the pET30b expression vector (Novagen) using T4 DNA ligase (ThermoFisher Scientific). ..

    Expressing:

    Article Title: Structural and Functional Investigation of the Ag(+)/Cu(+) Binding Domains of the Periplasmic Adaptor Protein SilB from Cupriavidus metallidurans CH34.
    Article Snippet: Silver ion resistance in bacteria mainly relies on efflux systems, and notably on tripartite efflux complexes involving a transporter from the resistance−nodulation−cell division (RND) superfamily, such as the SilCBA system from Cupriavidus metallidurans CH34.. The periplasmic adaptor protein SilB hosts two specific metal coordination sites, located in the N-terminal and C-terminal domains, respectively, that are believed to play a different role in the efflux mechanism and the trafficking of metal ions from the periplasm to the RND transporter.. On the basis of the known domain structure of periplasmic adaptor proteins, we designed different protein constructs derived from SilB domains with either one or two metal binding sites per protein chain.

    Article Title: Method for detecting pancreatic cancer
    Article Snippet: .. The plasmid that matched the target sequence was treated with NdeI and KpnI restriction enzymes, the resultant was purified using a QIAquick Gel Extraction Kit, and the target gene sequence was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with NdeI and KpnI restriction enzymes. ..

    Article Title: Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB
    Article Snippet: The amplified PCR product was cloned in the pJET1.2/blunt vector (ThermoFisher Scientific) prior to sequencing. .. The lasB gene was then excised by digesting with Nde I and Xho I and cloned into the pET30b expression vector (Novagen) using T4 DNA ligase (ThermoFisher Scientific). ..

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).

    Article Title: Method for detecting cancer using CAPRIN-1 as a marker
    Article Snippet: .. Then the gene sequence of interest was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with Nde I and Kpn I restriction enzymes. .. A His tag-fused recombinant protein can be produced using the vector.

    Plasmid Preparation:

    Article Title: Structural and Functional Investigation of the Ag(+)/Cu(+) Binding Domains of the Periplasmic Adaptor Protein SilB from Cupriavidus metallidurans CH34.
    Article Snippet: Silver ion resistance in bacteria mainly relies on efflux systems, and notably on tripartite efflux complexes involving a transporter from the resistance−nodulation−cell division (RND) superfamily, such as the SilCBA system from Cupriavidus metallidurans CH34.. The periplasmic adaptor protein SilB hosts two specific metal coordination sites, located in the N-terminal and C-terminal domains, respectively, that are believed to play a different role in the efflux mechanism and the trafficking of metal ions from the periplasm to the RND transporter.. On the basis of the known domain structure of periplasmic adaptor proteins, we designed different protein constructs derived from SilB domains with either one or two metal binding sites per protein chain.

    Article Title: Method for detecting pancreatic cancer
    Article Snippet: .. The plasmid that matched the target sequence was treated with NdeI and KpnI restriction enzymes, the resultant was purified using a QIAquick Gel Extraction Kit, and the target gene sequence was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with NdeI and KpnI restriction enzymes. ..

    Article Title: Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB
    Article Snippet: The amplified PCR product was cloned in the pJET1.2/blunt vector (ThermoFisher Scientific) prior to sequencing. .. The lasB gene was then excised by digesting with Nde I and Xho I and cloned into the pET30b expression vector (Novagen) using T4 DNA ligase (ThermoFisher Scientific). ..

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).

    Article Title: Method for detecting cancer using CAPRIN-1 as a marker
    Article Snippet: .. Then the gene sequence of interest was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with Nde I and Kpn I restriction enzymes. .. A His tag-fused recombinant protein can be produced using the vector.

    other:

    Article Title: Three-Dimensional Structure of N-Terminal Domain of DnaB Helicase and Helicase-Primase Interactions in Helicobacter pylori
    Article Snippet: The PCR product was subsequently digested with SalI restriction enzyme and cloned in pET30b (Novagen, Madison, WI, USA) expression vector at SalI site.

    Article Title: Functional characterization and transcriptional analysis of galE gene encoding a UDP-galactose 4-epimerase in Xanthomonas campestris pv. campestris.
    Article Snippet: After sequence confirmation, the fragment as excised from pTgalE and cloned into the pET30b expression ector (Novagen, Gibbstown, NJ, USA), giving pETgalE. pETgalE as used as a template for recombinant GalE protein production o express the cloned galE with the S30 T7 high-yield protein xpression system from Promega, following the manufacturer’s nstructions.

    Article Title: Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps)
    Article Snippet: All genes expressing the Vaps’ core region (see supplementary data for sequences) were cloned into pET30b or pET26a expression vectors (Novagen) with all proteins having a C-terminal His6-tag (Novagen).

    Sequencing:

    Article Title: Method for detecting pancreatic cancer
    Article Snippet: .. The plasmid that matched the target sequence was treated with NdeI and KpnI restriction enzymes, the resultant was purified using a QIAquick Gel Extraction Kit, and the target gene sequence was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with NdeI and KpnI restriction enzymes. ..

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).

    Article Title: Method for detecting cancer using CAPRIN-1 as a marker
    Article Snippet: .. Then the gene sequence of interest was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with Nde I and Kpn I restriction enzymes. .. A His tag-fused recombinant protein can be produced using the vector.

    Purification:

    Article Title: Method for detecting pancreatic cancer
    Article Snippet: .. The plasmid that matched the target sequence was treated with NdeI and KpnI restriction enzymes, the resultant was purified using a QIAquick Gel Extraction Kit, and the target gene sequence was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with NdeI and KpnI restriction enzymes. ..

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).

    Gel Extraction:

    Article Title: Method for detecting pancreatic cancer
    Article Snippet: .. The plasmid that matched the target sequence was treated with NdeI and KpnI restriction enzymes, the resultant was purified using a QIAquick Gel Extraction Kit, and the target gene sequence was inserted into a pET30b expression vector (Novagen) for Escherichia coli treated with NdeI and KpnI restriction enzymes. ..

    Amplification:

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).

    Introduce:

    Article Title: Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1
    Article Snippet: .. The cDNA of human CRBP2 was purchased from Origene Technologies and amplified by polymerase chain reaction (PCR) using the following primers: forward –GCAGATCATATGACAAGGGACCAGAATGGAACC and reverse –CGTCTACTCGAGTCCCGGGGATCCACGCGGAACCAGTTTCTTTTTGAACACTTGACGG to subclone into pET30b expression vector (Millipore Sigma), thereby modifying the C-terminal sequence to introduce thrombin cut site that allowed for removal of a 6x histidine tag from the purified protein. .. The recombinant protein was expressed in the BL21 (DE3) E. coli strain (New England Biolabs).



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